CD13 抗体 (FITC)
Quick Overview for CD13 抗体 (FITC) (ABIN5541585)
抗原
See all CD13 (ANPEP) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- This antibody reacts with mouse CD13.
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纯化方法
- Protein G agarose
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免疫原
- Murine dendritic cells isolated from C57BL/6 mice
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亚型
- IgG2b
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anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 668-967) antibody
ANPEP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 344-444) antibodyANPEP 适用: 人, 小鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 34-219) antibodyANPEP 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibodyANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal WM15 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibodyANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP, Func 宿主: 小鼠 Monoclonal WM15 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) (AA 781-967) antibodyANPEP 适用: 人 FACS, ELISA 宿主: 小鼠 Monoclonal 1C7D7 unconjugated
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (Biotin)ANPEP 适用: 人, Non-Human Primate FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal WM15 Biotin
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (FITC)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 FITC
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (PE)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 PE
anti-Alanyl (Membrane) Aminopeptidase (ANPEP) antibody (PerCP)ANPEP 适用: 人, Non-Human Primate FACS 宿主: 小鼠 Monoclonal WM15 PerCP
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应用备注
- Flow cytometry: 10 μg/m (final concentration). For details see protocol below.
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实验流程
- Flow cytometric analysis for floating cells We usually use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2 % fetal calf serum (FCS) and 0.1 % NaN 3]. 2) Resuspend the cells with washing buffer (5x10 6 cells/mL). 3) Add 50 μ L of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute at room temperature (20~25 o C). Remove supernatant by careful aspiration. 4) Add 10 μ L of normal goat serum containing 1 mg/mL normal human IgG and 0.1 % NaN 3 to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature. 5) Add 40 μ L of the primary antibody at the concentration of as suggest in the APPLICATIONS diluted in the washing buffer. Mix well and incubate for 30 minutes at room temperature. 6) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at room temperature. Remove supernatant by careful aspiration. 7) Resuspend the cells with 500 μ L of the washing buffer and analyze by a flow cytometer. (Positive controls for Flow cytometry JAWSII, C2C12)
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- 0.09 % NaN 3 Stabilizers: 1 % BSA
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储存条件
- 4 °C
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储存方法
- Store at 2-8 °C. Shelf life: one year from despatch.
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有效期
- 12 months
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- CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))
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别名
- cd13
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背景
- CD13 is a myeloid differentiation molecule expressed on committed myeloid progenitors, granulocytes, monocytes, and leukemic cells of myeloid origin. It is also expressed on non-hemopoietic cells including fibroblasts, renal proximal tubule, and small intestine brush-border membrane. The gene for CD13 has been cloned the cDNA sequence shows that CD13 is the metalloprotease aminopeptidase N. Biochemical studies have shown that CD13 is a 150 kDa glycoprotein, which exists as a 130 kDa intracellular precursor form. This 130 kDa precursor molecule is pos ttranslationally modified in the Golgi apparatus to produce the 150 kDa mature cell surface form of the molecule.
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UniProt
- P97449
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途径
- Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones
抗原
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